Finnzymes Application Notes
As you will see below, we have largely gathered data and protocols to demonstrate PCR results from a wide variety of starting materials without DNA purification, extraction or isolation.
Finnzymes' Phusion and Phire DNA Polymerases, due to their unique structure, are highly robust and tolerant of many PCR inhibitors. This resilience provides efficient amplification from many difficult samples. Note that PCR from an unpurified starting material often requires more optimization than standard PCR reactions from purified DNA and may not always be suitable for all applications. So, please read these protocols carefully when planning your direct PCR experiments and contact us with questions.
PCR directly from blood |
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Phusion® Blood Direct PCR Kit is designed for amplification of DNA directly from whole blood. |
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| See the product page for examples and additional information | ||
| Application note: Direct PCR from blood preserved on Whatman FTA® and 903® Cards | ||
| Application protocol: PCR-RFLP directly from blood using Phusion® Blood Direct PCR Kit | ||
PCR directly from plants |
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NEW! Phire® Plant Direct PCR Kit includes a complete set of optimized reagents, sampling tools and control primers for convenient amplification of plant DNA. | |
| See the product page for examples and additional information. | ||
| Which plant materials work with Phire Plant Direct PCR Kit? | ||
PCR directly from a wide range of tissue |
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NEW! Phire® Animal Tissue Direct PCR Kit is a complete kit for for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects | |
| See the product page for examples and additional information. | ||
| Which materials work with Phire Tissue Direct PCR Kit? | ||
PCR directly from formalin-fixed paraffin-embedded (FFPE) tissues |
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Application note: PCR from FFPE tissues without DNA extraction using Phusion® DNA Polymerase | |
| Gel images | ||
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Amplification of whole human mitochondrial DNA
Phusion™ High-Fidelity DNA Polymerases:
A PCR method for amplification of whole human mitochondrial DNA
PCR protocol for DyNAzyme II™ DNA Polymerase on the fast Piko™ Thermal Cycler
High Temperature PCR with DyNAzyme™ II DNA Polymerase
Provides Fast and Efficient Results
