Phi6 RNA Replicase
RNA replicase for a wide range of templates.Ordering information
Phi6 RNA Replicase |
Product Number |
Units | Price |
Order |
|
|---|---|---|---|---|---|
| F-611S | 60 U (1 U / µl) | $80 |
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| *Please contact us for large volume quotes or OEM oportunities | F-611L | 300 U (1 U / µl) | $360 |
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Description
The Phi6 RNA Replicase is a modified version of the protein P2 from bacteriophage Phi6. This novel RNA-dependent RNA polymerase catalyzes the synthesis of a full-length complementary RNA strand initiating from the 3’ terminus of a single-stranded RNA. The polymerase does not require any oligonucleotide primer for the initiation. Due to a unique modification, the Phi6 RNA Replicase displays relatively low template specificity and it is therefore capable of replicating a great variety of RNA templates, as well as denatured DNA that contains a recognition sequence for the Phi6 RNA Replicase at its 3’ terminus.
Applications
- Replication of ssRNA to dsRNA form
- Synthesis and amplification of dsRNA using
dsDNA as template
Detailed protocol - For producing larger amounts of dsRNA, we recommend the Replicator RNAi Kit
Contents
Phi6 RNA Replicase is supplied with 10x Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution. 1x buffer contains: 50 mM Tris-HCl (pH 8.75 at 21°C), 50 mM NH4Ac.
Storage buffer
50 mM Tris-HCl (pH 8.0 at 25°C), 0.1 mM EDTA, 100 mM NaCl, 0.1 % Triton® X-100 and 50 % glycerol.
| Submit Orders To: orders@finnzymes.com or fax to 1-617-245-1962 |
| Origin: Portsmouth, NH USA |
| Costs: All shipping costs will be prepaid and added to invoice |
| Shipping: All orders will be shipped with two-day service unless otherwise specified |
| Terms: Orders are shipped FCA Origin |
