Phire^® Hot Start DNA Polymerase

Speed and specificity for routine and high-throughput PCR.

Ordering information

Phire™ Hot Start DNA Polymerase	Product Number	Size	Price	Order
	F-120S	200 reactions in 50 µl	N/A*
	F-120L	1000 reactions in 50 µl	N/A*
Phire™ Plant Direct PCR Kit	Product Number	Size	Price	Order
	F-130S	200 reactions in 50 µl	N/A*
Phire® Animal Tissue Direct PCR Kit	Product Number	Size	Price	Order
	F-140	200 reactions in 50 µl	N/A*
DNARelease™ Additive	Product Number	Size	Price	Order
	F-355	3 x 100 µl DNARelease Additive 3 x 1 ml Gel loading dye	$90

Description

Finnzymes’ Phire® Hot Start DNA Polymerase is a novel PCR enzyme that outperforms every Taq-based hot start polymerase on the market. This is a result of the polymerase’s DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, our unique hot start technology allows complete reactivation of Phire Hot Start DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymerase an ideal solution for routine and high throughput PCR applications. Phire DNA Polymerase delivers superior performance in all thermal cyclers, both conventional and fast instruments such as the Piko® Thermal Cycler from Finnzymes.

Figure 1. Phire Hot Start DNA Polymerase is constructed by fusing a DNA polymerase (orange) and a small dsDNA-binding protein (yellow). This technology increases the processivity of the polymerase and improves its overall performance.

Advantages

Quick hot start: Zero-time reactivation
Fast enzyme: Amplify three times faster than with hot start Taq
High yields: Abundant products due to high efficiency
Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq

Quick hot start

Phire Hot Start DNA Polymerase utilizes Affibody® inactivation technology, which reversibly inactivates the DNA polymerase at room temperature. At polymerization temperature the Affibody ligand is released from the DNA polymerase. This completely eliminates the need for a DNA polymerase reactivation step in cycling protocol, thus speeding up PCR even more.

Fast PCR with high yields

The DNA-binding domain of Phire Hot Start DNA Polymerase stabilizes the polymerase-DNA complex during extension steps in PCR. This enables the polymerase to amplify DNA three times faster than standard Taq DNA polymerase. The high efficiency also results in abundant yields.