Phire^® Animal Tissue Direct PCR Kit

For amplification of DNA directly from animal tissues.

Ordering Information

Phire® Animal Tissue Direct PCR Kit	Product Number	Size		Price
	F-140	200 reactions in 50 µl		$285

Description

Finnzymes’ Phire® Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. No DNA isolation is required prior to PCR. The kit is based on our Phire® Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in unpurified animal tissues. This kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools and DNARelease™ Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers that can be used with numerous animal species. When combined with Finnzymes’ Piko® Thermal Cycler and UTW® vessels, PCR protocols can be completed in as little as 30 minutes.

Advantages

No need for DNA extraction
Phire Hot Start II DNA Polymerase delivers high inhibitor tolerance, specificity and high yields
Fast and simple protocols require minimal hands-on time
Convenient sampling tools included in the kit
Very little sample material required
Compatible with tissues from various animal species, including mice, fish and insects (see complete list of tested animal tissues here)

Two alternative protocols suitable for various purposes

In the direct protocol, a small piece of animal tissue is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1 µl of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample, for example.

Plant Direct PCR protocols

Fast and reliable PCR from various tissue samples from a number of species

Click pictures for a full-sized image

Direct PCR from various animal tissues	Genotyping F2 transgenic mice

Kit components

Kit component	F-140
Phire® Hot Start DNA Polymerase	200 µl
2x Phire® Animal Tissue PCR Buffer (includes dNTPs and MgCl2)	5 x 1 ml
Control primer mix	40 µl
Dilution Buffer	5 ml
DNARelease™ Additive	3 x 100 µl
Gel Loading Dye	3 x 1 ml
Harris Uni-Core™ 0.50 mm	1
Harris Cutting Mat™	1