Phire^® Plant Direct PCR Kit

For amplification of DNA directly from plant material.

Ordering Information

Phire™ Plant Direct PCR Kit	Product Number	Size		Price
	F-130S	200 reactions in 50 µl		$225

Description

Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start II DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant material. The kit includes a complete set of optimized reagents, sampling tools and control primers making it an easy and convenient choice for amplification of plant DNA. When combined with Finnzymes’ Piko® Thermal Cycler and UTW® vessels, PCR protocols can be completed in as little as 30 minutes.

Advantages

No need for DNA extraction
Phire Hot Start DNA Polymerase features high inhibitor tolerance and allows specificity with abundant yields
Fast and simple protocols require minimal hands-on time
Convenient sampling tools included in the kit
Only very little sample material required
Tested with leaves and seeds from a wide variety of plant species (see list here)

Two short protocols for various needs

In the direct protocol, a small piece of plant material is placed directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons , and for assays where several DNA fragments are amplified from the same sample.

Plant Direct PCR protocols

Efficient PCR from various plant leaves and seeds

Click pictures for a full-sized image

Direct PCR from plant leaves	Direct PCR from plant seeds

Which plant materials work with Phire Plant Direct PCR Kit?

Kit components

Kit component	F-130
Phire® Hot Start DNA Polymerase	200 µl
2x Phire® Plant PCR Buffer (includes dNTPs and MgCl₂)	5 x 1 ml
Control primer mix	40 µl
Dilution Buffer	5 ml
Harris Uni-Core™ 0.50 mm	1
Harris Cutting Mat™	1