Phusion^® Bacterial Profiling Kit

For easy profiling of bacterial populations.

Ordering information

Description

Finnzymes’ Phusion® Bacterial Profiling Kit enables fast profiling and characterization of bacterial populations. The profiling method is based on terminal restriction fragment length polymorphism (T-RFLP) and it is suitable for use with any sample material containing bacteria. Current examples include human, animal, food, and environmental samples. The Phusion Bacterial Profiling Kit includes a complete set of reagents for PCR and subsequent endonuclease digestion. The kit comes with a password for a web database of T-RFLP peak values, which contains more than 60,000 bacterial targets.

Application fields

• Monitoring bacterial changes in food and dairy industry
• Animal diagnostics of bacteria in healthy and diseased animals
• Environmental research
• Forensic comparison of soils
• Hospital hygiene, etc

Short and simple protocol

The Phusion Bacterial Profiling Kit includes a complete set of reagents for PCR and subsequent endonuclease digestion.

1. PCR with differentially labeled fluorescent primers   2. Endonuclease digestion   3. Capillary electrophoresis The T-RFLP electropherogram obtained with the Phusion Bacterial Profiling Kit contains two peaks for each sample representing the differentially labeled restriction fragments.   4. Characterization of the peaks using tailor-made database Access to a regularly updated database of T-RFLP peak values is included with the purchase of the Phusion® Bacterial Profiling Kit. Just log in and type the two peak values in the database. The database lists the bacterial species or subgroups matching to your search. Click here to see a screenshot of the database

Advantages

• Identifies the entire community at once (unlike sequencing methods)
• Can identify bacteria that cannot be cultured
• Robust PCR even with impure samples
• Easy-to-use mixes reduce risk of contamination
• No DNA purification needed for endonuclease digestion
• No false peaks in T-RFLP profile
• Access to a regularly updated web database of T-RFLP peak values

Kit components

Storage and stability

Recommended storage temperature -20°C. Stable for six months from the packaging date