Phusion® Blood Direct PCR Kit
For amplification of DNA directly from whole blood.
Ordering information
| Phusion™ Blood Direct PCR Kit | Product Number |
Size |
Price |
|
|---|---|---|---|---|
F-547S |
100 Reaction in 20 µl volume |
$95 |
||
F-547L |
500 Reactions in 20 µl volume |
$380 |
||
Description
Finnzymes’ Phusion® Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The kit utilizes a modified Phusion® Hot Start High-Fidelity DNA Polymerase. This highly processive and robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even at 40 % blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples with different anticoagulants, storage conditions and from various species.
Advantages
- No need for DNA extraction
- Allows up to 40 % blood in the PCR reaction
- Tolerates heparin, citrate, and EDTA, and is compatible with blood stored on various types of filter papers
- Modified Phusion Hot Start DNA Polymerase gives specific and accurate results with high yields and short protocol times
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 |
 |
| Superior performance even at very high blood concentrations | Robust amplification from a wide variety of template sources |
Kit components
Kit component | F-547S | F-547L |
|---|---|---|
Phusion® Blood DNA Polymerase |
40 µl |
200 µl |
2 x Phusion® Blood PCR Buffer (includes dNTPs and MgCl2) |
1 ml |
5 x 1 ml |
50 mM EDTA |
0.5 ml |
0.5 ml |
50 mM MgCl2 |
1.5 ml |
1.5 ml |
100 % DMSO |
0.5 ml |
0.5 ml |
Universal Control Primer Mix |
40 µl |
40 µl |
Storage and stability
Recommended storage temperature -20°C. Stable for one year from the packaging date
