Part of Thermo Fisher Scientific

Tm Calculator

This application calculates the Tm for a primer, and gives instructions on how to dilute the primer to a desired concentration.
 How to use this calculator

The application requires Java Virtual Machine.
  Download Java

Important notes

  • If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.
  • To determinine annealing temperature for PCR, read these instructions.

* Breslauer et al., Proc. Nat. Acad. Sci. 83, 3746-50 (1986)
** SantaLucia J., Proc. Nat. Acad. Sci. 95: 1460-1465 (1998)

Finnzymes, Inc. - Massachusetts, USA - Tel. 1-800-993-1283 - Fax 1-617-245-1962 - info@finnzymes.com