Tm Calculator

This application calculates the Tm for a primer, and gives instructions on how to dilute the primer to a desired concentration.

The application requires Java Virtual Machine.
Download Java

Important notes

If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.
To determinine annealing temperature for PCR, read these instructions.

*	Breslauer et al., Proc. Nat. Acad. Sci. 83, 3746-50 (1986)
**	SantaLucia J., Proc. Nat. Acad. Sci. 95: 1460-1465 (1998)